CAS号:113694-64-7
品名:Fura-2五钾盐Fura2pentapotassiumsalt
中文别名:FURA2五钾盐;2-[6-[双(羧甲基)氨基]-5-[2-[2-[双(羧甲基)氨基]-5-甲基苯氧基]乙氧基]-2-苯并呋喃基]-5-恶唑羧酸五钾盐;
英文别名:Fura-2,pentapotassiumsalt;FURA2PENTAPOTASSIUMSALT;
分子式:C29H22K5N3O14
分子量:831.988
精确质量:830.929
Psa:264.76
MDL号:MFCD00083328
PubChem号:24894731
密度:
沸点:983.5ºC at 760 mmHg
闪点:548.6ºC
储存条件:-20ºC
蒸汽压:0mmHg at 25°C
简介:Fura-2,anaminopolycarboxylicacid,isaratiometricfluorescentdyewhichbindstofreeintracellularcalcium.Itwasthefirstwidelyuseddyeforcalciumimaging,andremainsverypopular.Fura-2isexcitedat340nmand380nmoflight,andtheratiooftheemissionsatthosewavelengthsisdirectlyrelatedtotheamountofintracellularcalcium.Regardlessofthepresenceofcalcium,Fura-2emitsat510nmoflight.Theuseoftheratioautomaticallycancelsoutconfoundingvariables,suchasvariabledyeconcentrationandcellthickness,makingFura-2oneofthemostappreciatedtoolstoquantifycalciumlevels.Thehighphotonyieldoffura-2allowedthefirstrealtime(videorate)measurementsofcalciuminsidelivingcellsin1986.Morerecently,genetically-encodedcalciumindicatorsbasedonspectralvariantsofthegreenfluorescentprotein,suchasCameleons,havesupplementedtheuseofFura-2andothersmallmoleculedyesforcalciumimaging,butFura-2remainsfaster.
荧光探针,标记,颗粒和染色