CAS:123632-39-3
123632-39-3
CAS号：123632-39-3
品名：荧光钙探针Fluo-3Fluo-3
中文别名：荧光钙探针Fluo-3
英文别名：FLUO3;
分子式：C36H30Cl2N2O13
分子量：769.535
精确质量：768.112
Psa：224.58
密度：1.66 g/cm3
沸点：1056ºC at 760 mmHg
闪点：592.4ºC
储存条件：-20ºC
简介：Fluo-3isafluorescenceindicatorofintracellularcalcium(Ca2+).ItisusedtomeasureCa2+insidelivingcellsinflowcytometryandconfocallaserscanningmicroscopyusingvisiblelightexcitation(compatiblewithargonlasersourcesoperatingat488nm).Fluo-3isanessentiallynonfluorescentcompound,butuponbindingofCa2+itsfluorescenceincreasessharplywithanemissionmaximumat525nmsuitableforconventionallyuseddetectorsdesignedforfluoresceinisothiocyanate(FITC)measurements.ThislargechangeinfluorescencecoupledwithagoodyieldofphotonsprovidesveryhighcontrastwhichallowedthedetectionofmicroscopicCa2+releaseeventsinsidecellscalled\"Calciumsparks\".Whereasthesaltsoffluo-3areunabletopenetratecells,loadingcanbeachievedusingitsacetoxymethyl(AM)esterderivative.Onceinsidethecell,unspecificesterasescleavetheestereffectivelytrappingfluo-3.
荧光探针，标记，颗粒和染色
